Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53 (2025)

Bayaman, R. Y., Alkay, Z., Ispirli, H., Arioglu-Tuncil, S., Dere, S., Can, H., Alvarez Gonzales, M. A., Sagdic, O., Lindemann, S. R., Tuncil, Y. E. (2025). Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53. Fermentation, 11(6), Article 324. https://doi.org/10.3390/fermentation11060324

Bayaman, Rabia Yusra ; Alkay, Zuhal ; Ispirli, Humeyra et al. / Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53. In: Fermentation. 2025 ; Vol. 11, No. 6.

@article{29d8fc75845443e7ab72df6700fc6e6a,

title = "Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53",

abstract = "The production of novel oligosaccharides with potential prebiotic effects is of interest to expand the current market and explore the effectiveness of new functional carbohydrate forms. The utilization of glucansucrases is a cost-effective and environmentally friendly biotechnological strategy for producing novel gluco-oligosaccharides through acceptor reactions. In this study, an active glucansucrase (GS53) was used to produce gluco-oligosaccharides via its acceptor reactions with glucose, maltose, and maltotriose, and these oligosaccharides were tested in terms of structure and their gut microbiome modulatory effects. The formations of oligosaccharides were monitored by TLC analysis, and GS53 was active for the three acceptors but not for the other sugars tested. The structural characterization of the gluco-oligosaccharides by 1H NMR analysis revealed the glycosylation of each acceptor with α-(1 → 3) and α-(1 → 6) linkages, whereas LC-MS analysis demonstrated the formations of DP 8, DP 7, and DP 6 oligosaccharides with acceptors maltose, maltotriose, and glucose, respectively. In vitro fecal fermentation analysis, in which microbial short-chain fatty acids (SCFAs) and microbial compositional changes were assessed using gas chromatography and 16S rRNA sequencing, respectively, demonstrated that the gluco-oligosaccharides formed SCFAs—particularly propionate and butyrate—at levels comparable to those observed with inulin, a well-established prebiotic. Additionally, the gluco-oligosaccharides were found to promote the growth of Bifidobacterium adolescentis and Blautia OTUs, which are known to have important physiological functions beneficial to human health. Overall, these results demonstrate that gluco-oligosaccharides synthesized using GS53 through acceptor reactions exhibit prebiotic potentials and could be utilized in the future as dietary supplements as well as in the development of functional foods targeting colonic health.",

keywords = "acceptor reactions, butyrate, glucansucrases, gluco-oligosaccharides, gut microbiome, propionate, short chain fatty acids",

author = "Bayaman, {Rabia Yusra} and Zuhal Alkay and Humeyra Ispirli and Seda Arioglu-Tuncil and Sevda Dere and Hasan Can and {Alvarez Gonzales}, {Miguel Angel} and Osman Sagdic and Lindemann, {Stephen R.} and Tuncil, {Yunus Emre} and Enes Dertli",

note = "Publisher Copyright: {\textcopyright} 2025 by the authors.",

year = "2025",

month = jun,

doi = "10.3390/fermentation11060324",

language = "English",

volume = "11",

journal = "Fermentation",

issn = "2311-5637",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "6",

}

Bayaman, RY, Alkay, Z, Ispirli, H, Arioglu-Tuncil, S, Dere, S, Can, H, Alvarez Gonzales, MA, Sagdic, O, Lindemann, SR, Tuncil, YE 2025, 'Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53', Fermentation, vol. 11, no. 6, 324. https://doi.org/10.3390/fermentation11060324

Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53. / Bayaman, Rabia Yusra; Alkay, Zuhal; Ispirli, Humeyra et al.
In: Fermentation, Vol. 11, No. 6, 324, 06.2025.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53

AU - Bayaman, Rabia Yusra

AU - Alkay, Zuhal

AU - Ispirli, Humeyra

AU - Arioglu-Tuncil, Seda

AU - Dere, Sevda

AU - Can, Hasan

AU - Alvarez Gonzales, Miguel Angel

AU - Sagdic, Osman

AU - Lindemann, Stephen R.

AU - Tuncil, Yunus Emre

AU - Dertli, Enes

N1 - Publisher Copyright:© 2025 by the authors.

PY - 2025/6

Y1 - 2025/6

N2 - The production of novel oligosaccharides with potential prebiotic effects is of interest to expand the current market and explore the effectiveness of new functional carbohydrate forms. The utilization of glucansucrases is a cost-effective and environmentally friendly biotechnological strategy for producing novel gluco-oligosaccharides through acceptor reactions. In this study, an active glucansucrase (GS53) was used to produce gluco-oligosaccharides via its acceptor reactions with glucose, maltose, and maltotriose, and these oligosaccharides were tested in terms of structure and their gut microbiome modulatory effects. The formations of oligosaccharides were monitored by TLC analysis, and GS53 was active for the three acceptors but not for the other sugars tested. The structural characterization of the gluco-oligosaccharides by 1H NMR analysis revealed the glycosylation of each acceptor with α-(1 → 3) and α-(1 → 6) linkages, whereas LC-MS analysis demonstrated the formations of DP 8, DP 7, and DP 6 oligosaccharides with acceptors maltose, maltotriose, and glucose, respectively. In vitro fecal fermentation analysis, in which microbial short-chain fatty acids (SCFAs) and microbial compositional changes were assessed using gas chromatography and 16S rRNA sequencing, respectively, demonstrated that the gluco-oligosaccharides formed SCFAs—particularly propionate and butyrate—at levels comparable to those observed with inulin, a well-established prebiotic. Additionally, the gluco-oligosaccharides were found to promote the growth of Bifidobacterium adolescentis and Blautia OTUs, which are known to have important physiological functions beneficial to human health. Overall, these results demonstrate that gluco-oligosaccharides synthesized using GS53 through acceptor reactions exhibit prebiotic potentials and could be utilized in the future as dietary supplements as well as in the development of functional foods targeting colonic health.

AB - The production of novel oligosaccharides with potential prebiotic effects is of interest to expand the current market and explore the effectiveness of new functional carbohydrate forms. The utilization of glucansucrases is a cost-effective and environmentally friendly biotechnological strategy for producing novel gluco-oligosaccharides through acceptor reactions. In this study, an active glucansucrase (GS53) was used to produce gluco-oligosaccharides via its acceptor reactions with glucose, maltose, and maltotriose, and these oligosaccharides were tested in terms of structure and their gut microbiome modulatory effects. The formations of oligosaccharides were monitored by TLC analysis, and GS53 was active for the three acceptors but not for the other sugars tested. The structural characterization of the gluco-oligosaccharides by 1H NMR analysis revealed the glycosylation of each acceptor with α-(1 → 3) and α-(1 → 6) linkages, whereas LC-MS analysis demonstrated the formations of DP 8, DP 7, and DP 6 oligosaccharides with acceptors maltose, maltotriose, and glucose, respectively. In vitro fecal fermentation analysis, in which microbial short-chain fatty acids (SCFAs) and microbial compositional changes were assessed using gas chromatography and 16S rRNA sequencing, respectively, demonstrated that the gluco-oligosaccharides formed SCFAs—particularly propionate and butyrate—at levels comparable to those observed with inulin, a well-established prebiotic. Additionally, the gluco-oligosaccharides were found to promote the growth of Bifidobacterium adolescentis and Blautia OTUs, which are known to have important physiological functions beneficial to human health. Overall, these results demonstrate that gluco-oligosaccharides synthesized using GS53 through acceptor reactions exhibit prebiotic potentials and could be utilized in the future as dietary supplements as well as in the development of functional foods targeting colonic health.

KW - acceptor reactions

KW - butyrate

KW - glucansucrases

KW - gluco-oligosaccharides

KW - gut microbiome

KW - propionate

KW - short chain fatty acids

UR - http://www.scopus.com/inward/record.url?scp=105008902068&partnerID=8YFLogxK

U2 - 10.3390/fermentation11060324

DO - 10.3390/fermentation11060324

M3 - Article

AN - SCOPUS:105008902068

SN - 2311-5637

VL - 11

JO - Fermentation

JF - Fermentation

IS - 6

M1 - 324

ER -

Bayaman RY, Alkay Z, Ispirli H, Arioglu-Tuncil S, Dere S, Can H et al. Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53. Fermentation. 2025 Jun;11(6):324. doi: 10.3390/fermentation11060324